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high speed calcium imaging Maryline Beurg 1, Robert Fettiplace 2, Jong-Hoon Nam 2, and Anthony J. Ricci 3,* 1 INSERM U587, Université Victor Segalen Bordeaux 2, Hôpital Pellegrin, 33076 Bordeaux

High-speed in vivo calcium imaging reveals neuronal

2018-8-31  high-speed calcium imaging will facilitate optical studies of information processing in brain microcircuits. Neural circuits in the brain operate on the millisecond time scale via spatiotemporal patterns of neuronal spikes. Two-photon cal-cium imaging recently has enabled functional measurements from

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High-speed in vivo calcium imaging reveals neuronal

2010-4-18  Our RAPS mode enabled high-speed calcium imaging from neuronal populations. As an example, we measured spontaneous calcium transients in 55 L2/3 neurons at 298 Hz .

Spatiotemporal properties of high-speed calcium

We tested the hypothesis that high-speed calcium imaging would reveal calcium-mediated oscillations in dendritic compartments in synchrony with patch-clamp recorded oscillations during depolarizing current ramps. Patch-clamped 8- to 16-day-old rat PPN neurons (n 67 out of 121) were filled with Fura 2, Bis Fura, or OGB1/CHR.

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Spatiotemporal properties of high-speed calcium

Spatiotemporal properties of high-speed calcium oscillations in the pedunculopontine nucleus James Hyde,1 Nebojsa Kezunovic,1 Francisco J. Urbano,2* and Edgar Garcia-Rill1* 1Center for

Spatiotemporal properties of high-speed calcium

2019-10-29  We tested the hypothesis that high-speed calcium imaging would reveal calcium-mediated oscillations in dendritic compartments in synchrony with patch-clamp recorded oscillations during depolarizing current ramps. Patch-clamped 8- to 16-day-old rat PPN neurons (n = 67 out of 121) were filled with Fura 2, Bis Fura, or OGB1/CHR. This study also

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With high-speed, precisely-targeted, two-photon point imaging we measured both calcium rise and decay kinetics in spines and secondary dendrites in neocortical pyramidal neurons. We found that both rise and decay kinetics of changes in calcium-indicator fluorescence are about twice as fast in spines.

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High-Speed Brain wide 3D Calcium Imaging of Neuronal Activity with Sculpted Light Wide-field temporal focusing (WF-TEFO) is a two-photon imaging technique which is based on light-sculpting. It effectively decouples the parameters governing lateral size

Spatiotemporal properties of high-speed calcium

2019-10-29  We tested the hypothesis that high-speed calcium imaging would reveal calcium-mediated oscillations in dendritic compartments in synchrony with patch-clamp recorded oscillations during depolarizing current ramps. Patch-clamped 8- to 16-day-old rat PPN neurons (n = 67 out of 121) were filled with Fura 2, Bis Fura, or OGB1/CHR. This study also

High-speed in vivo calcium imaging reveals neuronal

2018-8-31  high-speed calcium imaging from neuronal populations We next implemented a random-access scanning mode for fast calcium measurements. Although the 10-mm AOD aperture size nominally supports point-to-point transitions within 15.4 µs (sound velocity 650

(PDF) Spatiotemporal properties of high speed

Spatiotemporal properties of high-speed calcium oscillations in the. pedunculopontine nucleus. James Hyde, 1. Nebojsa Kezunovic, 1. Francisco J. Urbano, 2 * and Edgar Garcia-Rill. 1 * 1.

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High Speed Two-Photon Imaging of Calcium

2007-10-24  With high-speed, precisely-targeted, two-photon point imaging we measured both calcium rise and decay kinetics in spines and secondary dendrites in neocortical pyramidal neurons. We found that both rise and decay kinetics of changes in calcium-indicator fluorescence are about twice as fast in

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OSA High-speed large scale volumetric calcium

High-speed large scale volumetric calcium imaging of neuronal circuits Alipasha Vaziri Author Information . Author Affiliations. Alipasha Vaziri The Rockefeller University, New York, NY & Research Institute of Molecular Pathology (IMP), Vienna, Austria Find other works by these authors .

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Spatiotemporal properties of high-speed calcium

2019-10-29  We tested the hypothesis that high-speed calcium imaging would reveal calcium-mediated oscillations in dendritic compartments in synchrony with patch-clamp recorded oscillations during depolarizing current ramps. Patch-clamped 8- to 16-day-old rat PPN neurons (n = 67 out of 121) were filled with Fura 2, Bis Fura, or OGB1/CHR. This study also

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Spatiotemporal properties of high-speed calcium

Spatiotemporal properties of high-speed calcium oscillations in the pedunculopontine nucleus Spatiotemporal properties of high-speed calcium oscillations in the pedunculopontine nucleus James Hyde, Nebojsa Kezunovic, Francisco J. Urbano, Edgar Garcia-Rill 2013-11-01 00:00:00 Downloaded from on June 16, 2014 Downloaded from on June 16, 2014 Journal of Applied Physiology publishes

High-Speed Calcium Imaging of Neuronal Activity

2021-2-11  High-Speed Calcium Imaging of Neuronal Activity Using Acousto-Optic Deflectors book. By Benjamin F. Grewe. Book Understanding Biophotonics. Click here to navigate to parent product. Edition 1st Edition. First Published 2015. Imprint Jenny Stanford Publishing. Pages 26.

High-speed-Calcium Imagine on Vimeo

This is "High-speed-Calcium Imagine" by Hamamatsu on Vimeo, the home for high quality videos and the people who love them.

OSA High-speed large scale volumetric calcium

High-speed large scale volumetric calcium imaging of neuronal circuits Alipasha Vaziri Author Information . Author Affiliations. Alipasha Vaziri The Rockefeller University, New York, NY & Research Institute of Molecular Pathology (IMP), Vienna, Austria Find other works by these authors .

[PDF] High‐Speed Multineuron Calcium Imaging

Conventional confocal and two‐photon microscopy scan the field of view sequentially with single‐point laser illumination. This raster‐scanning method constrains video speeds to tens of frames per second, which are too slow to capture the temporal patterns of fast electrical events initiated by neurons. Nipkow‐type spinning‐disk confocal microscopy resolves this problem by the use of

Localization of inner hair cell mechanotransducer

2017-5-5  Localization of inner hair cell mechanotransducer channels using high-speed calcium imaging Maryline Beurg1,4, Robert Fettiplace2,4, Jong-Hoon Nam2,4 & Anthony J Ricci3,4 Hair cells detect vibrations of their stereociliary bundle by activation of mechanically sensitive transducer channels.

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